Hyper-reactive mononuclear cells and neutrophils in chronic periodontitis
: Blackwell Synergy
Objectives: Stimulated mono- and polymorphonuclear cells from patients with
periodontitis have shown increased release of interleukin-1b (IL-1b) and oxygen
radicals, respectively. The aim was to study whether this hyper-reactivity could be
found both in mono- and polymorphonuclear cells from the same patient, and whether
there was a relation to the gene coding for IL-1b (IL-1b13953).
Material and Methods: Peripheral mononuclear cells from 14 non-smoking and
well-treated patients and pair-matched controls were incubated with opsonized
Staphylococcus aureus and lipopolysaccharide (LPS). Released IL-1b and tumour
necrosis factor (TNF)-a were determined with ELISA. Generation of oxygen radicals
from the Fcg-receptor-stimulated neutrophils was measured with chemiluminescence
and the polymorphism at IL-1b13953 was measured with polymerase chainreaction.
Results: The mononuclear cells from the patients released more IL-1b after
incubation with LPS (po0.001) and with bacteria (po0.05). The release of TNF-a
tended to be higher in the patient group. The peripheral neutrophils from the patients
generated more oxygen radicals (po0.06). We found no differences between the study
groups regarding the IL-1b13953 polymorphism.
Conclusion: The similarity in systemic inflammation between patients and controls
suggests that the increased release/generation of IL-1b and oxygen radicals from
peripheral leukocytes in periodontitis patients is of a constitutional nature and of
Daftar copy :
||Ruang Referensi - Perpustakaan FKIK
Diproses dalam : 0.15373611450195 detik