Periodontal therapy alters gene expression of peripheral blood monocytes
: Panos N. Papapanou
: Blackwell Synergy
Aims: We investigated the effects of periodontal therapy on gene expression of
peripheral blood monocytes.
Methods: Fifteen patients with periodontitis gave blood samples at four time points:
1 week before periodontal treatment (#1), at treatment initiation (baseline, #2), 6-week
(#3) and 10-week post-baseline (#4). At baseline and 10 weeks, periodontal status was
recorded and subgingival plaque samples were obtained. Periodontal therapy
(periodontal surgery and extractions without adjunctive antibiotics) was completed
within 6 weeks. At each time point, serum concentrations of 19 biomarkers were
determined. Peripheral blood monocytes were purified, RNA was extracted,
reverse-transcribed, labelled and hybridized with AffymetrixU133Plus2.0 chips.
Expression profiles were analysed using linear random-effects models. Further
analysis of gene ontology terms summarized the expression patterns into biologically
relevant categories. Differential expression of selected genes was confirmed by
real-time reverse transcriptase-polymerase chain reaction in a subset of patients.
Results: Treatment resulted in a substantial improvement in clinical periodontal status
and reduction in the levels of several periodontal pathogens. Expression profiling over
time revealed more than 11,000 probe sets differentially expressed at a false discovery
rate of o0.05. Approximately 1/3 of the patients showed substantial changes in
expression in genes relevant to innate immunity, apoptosis and cell signalling.
Conclusions: The data suggest that periodontal therapy may alter monocytic gene
expression in a manner consistent with a systemic anti-inflammatory effect.
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