MMP-13 and TIMP-1 determinations in progressive chronic periodontitis
: Marcela HernaÃ‚Â´ndez
: Blackwell Synergy
Matrix metalloproteinase (MMP)-13 is a collagenase involved in extracellular matrix
degradation either by its direct degradative effects or by processing bioactive
substrates. The aim of this study was to determine the levels of MMP-13 and tissue
inhibitor of metalloproteinase (TIMP)-1 in gingival crevicular fluid (GCF) and
gingival biopsies obtained from active and inactive sites during chronic periodontitis
Materials and Methods: This was a longitudinal study in which chronic periodontitis
patients with moderate to severe disease were included and followed until they
developed progression determined by the tolerance method. GCF samples were
obtained from periodontitis, active, inactive and healthy sites and additional gingival
biopsies were taken from active and inactive sites. MMP-13 and TIMP-1
determinations were carried out by immunodot blots and immunowestern blots.
Results: In progressive periodontitis, MMP-13 and TIMP-1 remained unchanged
between active and inactive sites, but as the TIMP-1 relative levels increased together
with MMP-13 elevation in inactive samples, an inverse correlation was observed in
active sites. Besides, MMP-13 was undetectable in healthy controls.
Conclusion: Chronic periodontitis is characterized by increased MMP-13 expression.
During disease progression, active sites tended to decrease TIMP-1 levels in
association with MMP-13 elevation.
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