TLR2 Arg753Gly, TLR4 Asp299Gly and Thr399Ile gene polymorphisms are not associated with chronic periodontitis in a Turkish population
: Blackwell Synergy
Aim: Toll-like receptor (TLR) gene polymorphisms could affect the hostÃ¢â‚¬â„¢s ability
to respond to microbial pathogens. In this caseÃ¢â‚¬â€œcontrol study, the association of TLR2
and TLR4 gene polymorphisms with chronic periodontitis (CP) was investigated.
Materials and Methods: Genomic DNA was obtained from the peripheral blood of 83
patients with CP and 106 periodontally healthy subjects. The TLR2 Arg753Gly,
Arg677Trp and TLR4 Asp299Gly, Thr399Ile gene polymorphisms were genotyped by
the polymerase chain reaction-restriction fragment length polymorphism method. The
data were analysed by a w2 test, logistic regression analysis and theMannÃ¢â‚¬â€œWhitney U test.
Results: The 753Gln allele was found in 6.1% of the CP patients as compared
with 6.6% in the control group (p40.05). The frequency of the 299Gly and 399Ile
allele was 2.4% and 1.8% in CP patients. For the healthy subjects, the frequency
was 2.8% for the 299Gly and 2.5% for the 399Ile allele (p40.05). None of the CP
patients or healthy subjects showed homozygosity for the TLR2 and TLR4 alleles.
Percentage of sites with bleeding on probing and plaque were significantly higher in
299Gly-positive patients compared with 299Gly-negative patients (po0.05).
Conclusion: These results showed that the TLR2 and TLR4 gene polymorphisms
studied are not associated with susceptibility to CP in Turkish patients.
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