MMP-2,MMP-9andTIMP-2gene polymorphisms in Chinese patients with generalized aggressive periodontitis
: Blackwell Synergy
Background: Aggressive periodontitis (AgP) has a genetic basis. It has been reported
that the functional gene polymorphisms of matrix metalloproteinase (MMP)-2, MMP-
9 and tissue inhibitor of metalloproteinase-2 (TIMP-2) alter their expressions in
transcriptional level and they are involved in the tissue destruction of periodontitis.
The study was carried out to analyse the association of functional polymorphisms in
MMP-2, MMP-9 and TIMP-2 with generalized AgP (G-AgP) in a Chinese population.
Material and Methods: The study population consisted of 79 Chinese patients with
G-AgP and 128 healthy controls. DNA was obtained from oral mucosa swab samples.
MMP-2 genotypes were determined by PCR-based denaturing high-performance
liquid chromatography analysis while MMP-9 and TIMP-2 genotypes were identified
by a PCR-based restriction fragment length polymorphism. w2 test after YatesÃ¢â‚¬â„¢
correction was used to investigate the possible association of the genotypes with the
Results: Although gene polymorphisms for MMP-2 and MMP-9 did not show
any association with the G-AgP, the analysis of the TIMP-2 418G to C gene
polymorphism revealed significant differences between the patients and controls.
Compared with controls, a significant increasing trend of TIMP-2 418C carrier in
the G-AgP patients occurred (p50.013).
Conclusion: It is suggested that the TIMP2 418G to C gene polymorphism is
associated with G-AgP in the Chinese subjects.
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