Total protein concentration and total bacterial loadasmeasuresof residual interproximal plaque in comparative clinical trials
: Nebojsa Milanovich, Jinling Wei,
: Blackwell Synergy
Aim: Establish total protein concentration and total bacterial load as quantitative
measures of residual interproximal plaque (IPP) in a clinical model designed to
evaluate oral hygiene interventions.
Material and Methods: This clinical model was a randomized, examiner and
laboratory technician-blinded, parallel-design study whereby levels of residual IPP
were compared for subjects using a manual toothbrush or a toothbrush1floss.
Differences between interventions were compared after 7 and 21 days of use. Protein
concentration was measured using 3-(4-carboxybenzoyl) quinoline-2-carboxaldehyde
in a fluorescence microplate format and bacterial load was assessed by quantitative
real-time PCR with universal primers specific for 16S rRNA and detected by SYBR
Green. ANCOVA was used to assess the statistical significance of the differences between
interventions while clinical relevance was evaluated by a statistical model described
by Man-Son-Hing et al. 2002.
Results: Ninety-three subjects completed the study. Significant differences between
interventions, using both outcome measures, were observed after 7 and 21 days.
The difference between interventions by total protein concentration were
further determined to be clinically relevant.
Conclusions: Only total protein concentration provided both statistically significant
and clinically relevant differences between two clinically distinct oral hygiene
interventions in this clinical model for evaluating IPP.
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